The Lumipulse analyzer from Fujirebio is a fully automated platform dedicated to high-precision immunoassays. Designed to meet the needs of clinical laboratories, the Lumipulse range utilizes CLIA (Chemiluminescent Immunoassay) technology, ensuring exceptional sensitivity and highly reliable results.

Key Features of the Lumipulse Analyzer:

• Advanced CLIA Technology: Lumipulse employs chemiluminescent immunoassays, offering highly sensitive and specific biomarker detection suitable for various clinical fields, including neurodegenerative diseases, oncology, and infectious diseases.
• High Performance and Speed: This analyzer is designed for high-throughput analysis while maintaining high precision and optimal reproducibility, enabling the processing of large sample volumes in short timeframes.
• Full Automation: The Lumipulse automates the entire analytical process, from sample preparation to result generation, minimizing manual intervention and reducing the risk of human error.
• Test Versatility: The Lumipulse range offers a wide variety of tests across multiple disciplines, including tumor markers, autoimmune diseases, infectious diseases, as well as cardiovascular and metabolic conditions.
• Ease of Use: With an intuitive interface and simplified reagent management, the analyzer is user-friendly and allows real-time monitoring of samples and results.

Assay Methodology:

The Lumipulse G1200 is a fully automated analyzer capable of performing immunoassays for multiple analytes in serum, plasma, or urine, utilizing chemiluminescence (CLIA). Depending on the assay, it uses either a sandwich-type immunometric method or a competitive method. The chemiluminescence process involves magnetic particles coated with an antigen or antibody, which are incubated with the sample and conjugated with alkaline phosphatase (ALP) and a luminescent chemical substrate. The luminescence produced by the immunocomplex is measured by a photometric detector.

There are two types of measurement methods: one-step and two-step, with an optional dilution step.

• One-step method: The sample, conjugate (ALP), and magnetic particles are incubated together. Magnetic particles are captured using a magnet, and unbound components are washed away. The substrate is then added, causing phosphorylation of ALP, which produces a light signal detected and measured.
• Two-step method: The sample and magnetic particles are incubated first. After washing away unbound components, the conjugate (ALP) is added during a second incubation. Another wash follows, and the substrate is added for luminescence measurement.
• Two-step method with dilution: The sample is first diluted with a sample diluent, and the process continues with the same incubation and washing steps as in the two-step method.

Clinical Applications:

The Lumipulse is widely used in diagnostic fields, including the screening of neurodegenerative diseases such as Alzheimer's, the detection of tumour markers, and the monitoring of infectious and cardiovascular diseases. Its ability to provide rapid and reliable results makes it an essential tool for laboratories aiming to improve workflow efficiency and diagnostic quality.

In summary, the Lumipulse from Fujirebio combines performance, reliability, and flexibility, offering an ideal solution for laboratories seeking to optimize their diagnostic capabilities while ensuring accurate and fast results.